Maresin1 alleviates liver ischemia/reperfusion injury by reducing liver macrophage pyroptosis

Abstract Background Cell pyroptosis has a strong proinflammatory effect, but it is unclear whether of liver macrophages exacerbates tissue damage during ischemia‒reperfusion (I/R) injury. Maresin1 (MaR1) anti-inflammatory and can suppress macrophage needs further study. Methods This study aimed to investigate MaR1 alleviate I/R injury by inhibiting pyroptosis. The effects on cell mitochondrial were studied dividing cells into control, hypoxia/reoxygenation, hypoxia/reoxygenation + groups. Knocking out RORa was used the mechanism which exert its protective effects. Transcriptome analysis, qRT‒PCR Western blotting analyze gene expression. Untargeted metabolomics techniques metabolite profiles in mice. Flow cytometry assess death damage. Results We first found that significantly reduced observed decreased Then, we discovered promotes oxidative phosphorylation, increases synthesis ATP, reduces generation ROS, decreases impairment membrane potential inhibits opening permeability transition pores. protecting mitochondria. Finally, exerts through activation nuclear receptor PI3K/AKT signaling pathway. Conclusions During injury, reduce reducing damage, thereby